Bioactivity assays of the complexes for calculating IC 50 were carried out with a SpectraMax 190 microplate reader (Molecular Devices, USA). Double-click the unknown device with the yellow warning icon. it should turn freely, without any friction. 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190 quantity. It may be named "Pothos". This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 2. . The ABS reader ranges from 340–850 nm in wavelength and the ABS Plus reader ranges from 190–1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Read - For the SpectraMax 190 and SpectraMax 340PC384, press to initiate the plate read when you operate the instrument in stand alone mode. Analysis of pro-inflammatory macrophage phenotypic response to NAM and RSV. The mixture was shaken and incubated for 30 min at room temperature in the dark. , China). The PathCheck sensor can extend the instrument linearity by a factor of three or more. Also refer to this Multimode Reader Comparison Chart. Molecular Devices technical support is available by phone and e-mail. EZ-Cytox (10 µL) was added to each of the wells of the plate and incubated for 1 h. Spectramax 190 Absorbance Microplate reader, included validation report, control software, 190-850 nm, Fully inspected and validated with Warranty. The dual-scanning capability can also be used to determine excitation and emission settings for new fluorescent probes. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard. Stray light. used. Protein concentrations were calculated using. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The VersaMax and SpectraMax 190 read 96-well microplates. The SpectraMax 190 reader is the tool for DNA analysis. The cell viability was normalised to the control group. Prior to the reading of each plate, the microplate reader was “blanked” with a. Auction Details Bio + Laboratory Equipment Expo. For customers outside the U. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. Three-dimensional Matrigel culture. The corrected absorbance is within 5% of the. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 313 reference gain check fail. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 0 out of 5 $ 4,499-5%. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. SpectraMax Gemini EM & FLEXstation (bottom read) 1. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. 4 mM −1 cm −1 for H 2 O 2. The patented multichannel optical design of the SpectraMax 190 Absorbance. Packed 25 plates per sleeve, 2 sleeves per case. Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaksIMI detection was carried out using the Eurofins Imidacloprid ELISA kit (Eurofins Abraxis, Warminster, PA, USA) following the manufacturer’s instructions for extraction and quantification of imidacloprid residue in leaf tissue and using a spectrophotometer (SpectraMax 190; Molecular Devices, San Jose, CA, USA). The reaction continued for 7 min and was subsequently stopped by adding 50 μL of 0. Molecular Devices SpectraMAX 190 Microplate Reader/Spectrophotometer, Tested. Both microplate and cuvette ports can be qualified. SOFTmax PRO. Type: Clear: Add to Cart. Biotek PowerWave XS Microplate Reader BioTek Synergy HTX Microplate Reader - Absorbance Only BioTek ELx808 Ultra Microplate Reader - Absorbance OnlySpectraMax Plus or SpectraMax 190 and SoftMax Pro Software automatically: •Make A 260 and A 280 measurements •Measure the optical pathlength of each sample with the PathCheck sensor •Normalize the absorbance values to a 1 cm pathlength •Calculate concentrations •Make specialized calculations (e. SpectraMax 190 and Spect FRET fluorescence, top- le-Mode Detection Fluorescence Sir and bottom-read, cell-based assay, lal injection in both 96- and 384-well lates, excellent sensitivity, Autorinse range, du microp 0+ pre-defined protocols; lac compatible based (visible 405-750 nm)The obtained mixture was reacted in a boiling water bath for 20 min, and then, cooled down immediately. The absorbance of the reaction mixture was recorded at 593 nm (SpectraMax 190, Molecular devices) after 5 min. Filter wheels can be shipped preloaded. Upgradeable modules are also available including western blot, cell imaging, and fast. Colorimetric reaction was stopped by adding 2 NH2SO4. . Glow-type luminescence provides an extended signal time window enabling batch processing of plates in screening assays;Colorimetric changes were measured using a SpectraMax 190 microplate spectrophotometer at 450 nm with a wavelength correction set at 620 nm. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Fluorescent Polarization: Solid black plates, as above. The PathCheck sensor can extend the instrument linearity by a factor of three or more. The VersaMax and SpectraMax 190 read 96-well microplates. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Add to cart. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Manufacturer: Molecular Devices; Model: SpectraMax 190 * Wavelength Selection Monochromator, tunable 1. It measures the optical density (OD) of samples in 96-wellSpectramax M Series, supplied by Danaher Inc, used in various techniques. . Offer Ends In: 44 Days 19: 59: 40. SpectraMax® Plus 384. Widen the range of quantitative experiments with a 190-1000 nm wavelength range. SSLLC offers a wide. 3. VC was used as the control substance. 2) If you wish to regulate the temperature inside the microplate reading cham-. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. #730. ®The optical density (OD) values of the bacterial cultures were measured at 595 nm in a spectrophotometer (SpectraMax 190; Molecular Devices, Sunnyvale, CA, USA). Total flavonoid content The total flavonoid content of the extracts was determined according to the method described by Herald et al. 3 Figures from 612 Publications Promega. For very concentrated samples, use aBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. For more information, please visit us at 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. Description. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe VersaMax and SpectraMax 190 read 96-well microplates. min −1. Description 1. e. Model: SpectraMax 250 Condition: Used. Optional tools for verifying the SpectraMax 190 reader performance include the SpectraTest ABS1 Absorbance Plate and IQ/OQ/PQ manual. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. In brief, 8-well chamber culture plates were coated with a thin layer of 8. , call 1-800-635-5577. Three low volume 24-well bottom slides. Theseinstrumentsmeasuretheopticaldensity(OD)ofsamplesatselected wavelengthsinasinglereadmode. 3 mmol) was added to a flask containing a 10 mL of methanol solution of HPPH (0. The SpectraMax® 190 can read as low as 2μL with the μMax Low Volume Plate. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 25% HF. We have 1 Molecular Devices SpectraMax 190 manual available for free PDF download: User Manual Molecular Devices SpectraMax 190 User Manual (55 pages) Microplate Spectrophotometers Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. For wavelengths in the UV range above 220 nm,. Customer Satisfaction is our number one goal All lab equipment in stock already tested and ships in 1-3 days• The TRIAX 180/190 models have four leveling feet and four foot pads. Protein concentrations were calculated using polynomial 2nd analysis from the protein standards. 1. To contact technical support by phone in the U. com. polynomial 2nd analysis from the protein standards. Features and Benefits Corning ® UV-Transparent Microplates are ideal for determining protein and/or nucleic acid concentrations. The 96-well plate was detected by a SpectraMax® 190 microplate reader (Molecular Devices, San Jose, USA) at 490 nm to obtain the relative cell viability. Exceptional performanceThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. The SpectraMax® and VersaMax™ require proper ventilation to keep their internal components cool. The SpectraTest® ABS1. Add to Cart Buy. Part # 0112-0085 Rev. Wavelength Bandwidth. Note: Due to. . Examples: fiPress carefully to engage,fl fiDo not press down. Absorption was measured at 450 nm 24 h after premix added using SpectraMax 190 (Molecular Devices, LLC, Sunnyvale, CA, USA) to produce a cell. Molecular Devices SpectraMax 190 Microplate Reader. Molecular Devices Spectramax 190 Absorbance Microplate Reader. Manufacturer: Molecular Devices; Model: SpectraMax 190; The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. It measures the optical density (OD) of samples in 96-well 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The decrease in absorbance due to decomposition of H 2 O 2 was monitored at 240 nm in a SpectraMax 190 microtitre plate-reader. Add to cart. Brand: Molecular. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Read Cuvette - For the SpectraMax Plus 384, press to initiate the sample read of the cuvette. SpectraMax and VersaMax . Performance Specifications . Italic and boldface type are used for emphasis. Price: $2,200. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toCommunity forums for Molecular Devices - SpectraMax M2 / M2e relating to Error #404? on LabWrench. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Wavelength range 190–1000 nm Wavelength selection Monochromator, tunable in 1. The soluble PAs were quantified by the DMACA method. . The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Additional models are available. See the installation guide or user guide. The cell pellets were lysed with 200 mmol/L NaOH, and the iron content was determined using the ferrozine assay. 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190. The SPECTRAmax 190 can accommodate standard 96-well microplates, strip wells, and Þlter-bottom microplates. $500. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. When the user applies a set temperature of 37°C, the top is heated to 37. Temperature control and shaking come as standard. The following steps provide a quick reminder of the basic operating proce- Overview dures required to read a microplate using the SPECTRAmax 190. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. The SpectraMax 190 reader is the tool for DNA analysis. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. See More Information. Microplate Readers. Minimize sample volume while retaining sensitivity with the SpectraDrop Micro-Volume Microplate. The SpectraMax 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. Multimode Readers Comparison Chart. I do that by turning the unit off, and turning the wheel. Prepare your i3/i3x instrument for shipment. Small volumes (5 μL, 50 mM) of compound solution, dissolved into DMSO, were added to the. to 11. absorbance readers. Molecular Devices SpectraMax 340PC384 Microplate Reader $ 4,000. com always has the largest. 1) Select the correct plate and the wells that you want to measure. Widen the range of quantitative experiments with a 190-1000 nm wavelength range. Page 18 VersaMax. Learn more about Services for SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. The multi-channel design truly mimics a dual-beam spectrophotometer. The SpectraMax 190 has a multichannel design for 96-well Molecular Devices SpectraMax 190 Temperature range: 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices SpectraMax 190 can be used to quantify DNA and perform endpoint, kinetic, and spectral scanning analyses. 02, 0. Add to Cart. book Page 1 Wednesday, July 7, 2010 2:09 PMSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. for every assay. 0 nm. Find many great new & used options and get the best deals for Molecular Devices SpectraMax 190 Absorbance Microplate Reader at the best online prices at eBay! Free shipping for many products!An ELISA reader (SpectraMax 190) was used to measure the absorbance of formazan solution at 490 nm. Microplate Readers Promega GloMax Plate Reader Multi-Detection. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Fig 2: Enzymatic response of synthetic compounds. ®{"general":{"total":5,"offset":0,"limit":5,"data":[{"key":"label","path":"path","name":"Name","identifier":"Identifier","wavelength-ranges":"Wavelength ranges. A. ®System (PerkinElmer) On The SpectraMax ID5 Reader. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm – 850 nm). Spectrophotometric assays were carried out using a SpectraMax 190 Microplate Reader (96-well flat bottom cell culture plate—Kasvi®). ®User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] mg/ml, 80 μl) was incubated at 25°C in the dark for 30 min, then the absorbance recorded at 517 nm (SpectraMax 190 Microplate Reader, USA). Optimized for fluorescence microplate readers. Standards were plotted using a log-log curve fit in SoftMax Pro Software. * dsDNA sensitivity specified for the following instruments: SpectraMax® 190 Readers, SpectraMax® Plus384 Readers,. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. 0 out of 5 $ 9,995 $ 9,495. The SpectraMax® 190 Absorbance Plate Reader has a temperature range of 190 - 850 nm, a wavelength accuracy of <±2. HI Wendy, I dont have a lot of expertise but I'd just suggest you to check that the filter wheel isn't touching the metal chassis. Example: [Drawer]. Wavelength Range: 190 - 850 nm. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. . 314 low lamp level warning. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 0 nm increments . It measures the optical density (OD) of samples in 96-wellPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. After incubation at 37 °C for 15 min, the resulting color reaction was read at 450 nm by a microplate reader (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA, USA) within 10 min, and the final IL-1 concentration was calculated using a 4-parameter logistic curve fit. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. Molecular Devices’ PathCheck® sensor technology. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. The inter- and intra-assay CV for the SAA analysis were less than 10%. The SpectraMax 190 reader is the tool for DNA analysis. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. The fresh whole blood was obtained from SD rats and anticoagulated with 3. Prepare your i3/i3x instrument for shipment. The SpectraMax® 190 Absorbance Microplate Reader is the ideal device for many life science applications, including DNA analysis. Examples: “Press carefully to engage,” “ Do not press down. Italic and boldface type are used for emphasis. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The multi-channel design truly mimics a dual-beam spectrophotometer. 2 nm 光度量范围 0-4. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 0 nm increments . In vitro blood test. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. SpectraMax® 340PC384 Microplate Reader for. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. You can also contact Technical Support by email. 1) Turn on the power switch of the SPECTRAmax 190 (located on the back panel). 00 0 bids 2d 20h. 00 . The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. . SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. 2 nm. The MIC was determined by the concentration where there was an obvious clear-cut decrease in the absorbance. High tolerance for contaminants. SpectraMax® 190. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The cooled mixture was centrifuged at 4000 rpm for 10 min, and the supernatant was collected. STAKMAX. The SpectraMax ® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. 0 nm. Fluorescence Microplate Reader Comparison Chart. 9000-0756. ; Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Table 1. It measures the optical density (OD) of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength. Microplate spectrophotometer (54 pages) Measuring Instruments Molecular Devices FlexStation II Operator's Manual. The UV-vis. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]®Plus384addstheabilitytoread cuvettes. What is a Roadtrek 190? Browse Roadtrek 190 RVs for sale on RvTrader. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . The SpectraMax 190 Microplate Reader is a high-performance spectrophotometer that can measure DNA absorbance across the microplate with a unique quartz fiber optic system and a PathCheck sensor. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. It measures the optical density (OD) of samples in 96-well Low-volume, high-throughput DNA and protein detection on the SpectraMax ABS Plus Microplate Reader. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Check Details. 078 μg/mL), and the results were expressed as μg Fe (II)/g of extract. . i3. Additionally, the SpectraMax 190 Microplate Reader can be seamlessly integrated with the StakMax Micropate Handling System through the. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Figure 1. to View Figures. Lipid accumulation in beige adipocytes treated with rosiglitazone or diosmin was detected by Oil Red O staining and measured at 520 nm by SpectraMax 190 microplate reader (Molecular Devices). spectramax plus 384 microplate reader, pathcheck sensor, spectramax plus 384 reader, softmax pro software, stakmax microplate stacker, spectratest validation package, microplate. Robust, high-value microplate readers that can run samples. Simplify and expedite experiments with SoftMax Pro. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. It measures the optical density (OD) of samples in 96-well0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The VersaMax and SpectraMax 190 read 96-well microplates. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Molecular Devices Spectramax 190 Absorbance Microplate Reader. Molecular Devices Spectramax 190 Microplate Reader Molecular Devices Spectramax 190 Microplate Reader Specifications. Temperature control and shaking come as standard. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. Our Price: $6,995. The narrow bandwidth provides greater measurement accuracy and linearity over the widest assay range, including DNA. SoftMax® Pro Software User Guide — 0112-0140 Rev. A layer of growth factor-reduced Matrigel was made for 3D cultures . TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate. Chitin weight was plotted against corresponding absorbance values resulting in a standard curve. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Perform the following steps to install the drivers: Start > Control Panel > System. A four‐parameter logistic function was used to fit the curve and determine unknown concentrations with the software. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. SpectraMax 190 Absorbance Microplate Reader. Narrow 2nm bandwidths deliver increased measurement accuracy and. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. A SOFTMAX® PRO SOFTWARE Version 5 for Mac® and Windows® User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. The absorbance was measured using ELISA analytical instruments SpectraMax 190 (Thermo Fisher Scientific, CA, United States) at a wavelength of 450 nm. D) units. 0 nm. The VersaMax and SpectraMax 190 read 96-well microplates. 7°C (gradient heating design). Free shipping. softmax pro software, spectramax paradigm, spectramax m5e/m5, walk-away automation, lab automation, fda 21 cfr oart 11, glp, gmp, stakmax Created Date: 1/11/2022 12:34:59 PM. Since this is an older reader, we recommend that new users choose our newer model i3 reader. Wavelength Bandwidth. S. The VersaMax and SpectraMax 190 read 96-well microplates. Temperature control and shaking come as standard. According to the manufacturer, the sensitivity of this assay is 5 ng/ml and has less than 1% cross. 8 ng/mL. A SOFTMAX® PRO SOFTWARE Version 5 for Mac® and Windows® User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. . #655801; Quartz: Hellma cat. Wavelength range 190 nm to 850 nm. The technology allows automatic adjustment of good absorbance to a tube equivalent path length of 1 cm, converted to 96 or 384 tubes. The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. All the measurements were taken in triplicate and the mean values were calculated. . Apr 21th, 2023Spectramax 190 Microplate ReaderThe SpectraMax 190 Reader Can Be Integrated With Molecular Devices’ StakMax® Microplate Stacker In A Matter Of Minutes And Begin Reading Microplates With Seven Mouse Clicks. 7 and HaCaT cells were pretreated with MAEO at 12. We assessed the expression of pro- and anti-inflammatory genes expressed by macrophages, including. SOFTmax PRO makes it possible to. The clearance of DPPH was calculated by the. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. The SpectraMax 190 Microplate Reader is a high-performance spectrophotometer that can measure DNA absorbance across the microplate with a unique quartz fiber optic system. ®This reaction was followed in vitro by absorbance at λ abs = 340 nm by spectrophotometry (SpectraMax 190, Molecular Devices) in UV-transparent 96-well plates (Costar, Corning) at 21 °C. Información para pedidos: Includes current version of SoftMax Pro Software. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. (volume is not critical because water does not absorb light appreciably between 190 and 900 nm) and read the plate. The final value was expressed as ng/g protein. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. We don't know when or if this item will be back in stock. S. used. The 293T cells were seeded into 6-well plates, using 330,000 cells. Add to Cart. GloMax ® Navigator System. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. (190 nm - 1000 nm). TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive measurementsofavarietyofanalytesacrossawiderangeofconcentrations. Spleen Cell. SpectraMax 190 Register or Sign In. 190-SC - SpectraMax 190 Microplate Reader with 1-Year Service Contract - SpectraMax 190 Absorbance Plate Reader, Molecular Devices - Each מותג: Molecular Devices 36,002. Slopes from the two curves were. 20 mM, 1 ml) and CPFE (5. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. 5–100 μg/mL for 1 h, followed by stimulation with LPS (0. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. ATP standard curves read on SpectraMax microplate readers. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Also for: Spectramax plus. Molecular Devices microplate spectrophotometer, mod SpectraMAX 190, s/n 02552, includes computer. Near bottom. Molecular Devices Spectrophotometer Spectra MAX 190. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. 1. For very concentrated samples, use a minimum…200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Web Information The Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Very small footprint suitable for tight space requirements. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Streamline GLP/GMP compliance. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax 190 Measuring Instruments. Optical density was measured at 570 nm with an automatic microplate spectrophotometer (SpectraMax 190, Sunnyvale, USA). SpectraMax ID5 Plate Reader (Molecular Devices) DescriptionCrystal violet was then dissolved using a 10% acetic solution, and the absorbance was read using SpectraMax 190 Microplate Reader (Molecular Devices) at 570 nm. ¨The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. We enable science by offering product choice, services, process excellence and our people make it happen. See SpectraMax® Microplate Reader User Manual Download Page. Not all manufacturers microplates are the same with regard to design, mate- rials, or conÞguration. Usage/Application: Lab. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Quercetin was used as the standard (1 mg/ml) solution in order to obtain the calibration curve using a series of concentrations, i. Examples: “Press carefully to engage,” “ Do not. Each sample has a discrete sample beam and reference. #3635 or Greiner cat. Cell Apoptosis Assay Following treatment, the apoptosis rate of H9c2 cells was analyzed by flow cytometry analyses using the Annexin V-FITC/PI Cell Apoptosis Detection Kit (Dojindo. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. The optical density was measured at 540 nm using a SpectraMax 190 microplate reader (Molecular Devices, San Jose, CA, USA). The patented multichannel optical design of the SpectraMax® 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate. The VersaMax and SpectraMax 190 read 96-well microplates. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual iii Labor and Parts To obtain warranty service during the applicable warranty period, you must take the product or de-liver the product properly packaged in the original shipping materials and carton to an authorized Molecular Devices Corporation service facility. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. All service plan coverage will end by December 31, 2028. Dual injectors for kinetic assays. Molecular Devices also offers a mass spectrometry-based solution for food safety testingSpectraMax - 190 - Microplate Reader by Molecular Devices, LLC. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. g. Description 1. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. 0 nm increments Wavelength bandwidth 2 nm Wavelength accuracy ±1. 315 can't find zero order.